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Reviewed by:. On Last modified: More Details. In particular, when the effects of high-protein diets are examined, casein as a protein source does not seem to be representative.
It can be questioned whether the observed beneficial. Protein-Protein Interactions: Methods for Detection and Analysis.
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Protein Expression. Monoclonal Antibody. Polyclonal Antibody. Recombinant Antibody. Protein Refolding. Protein Purification. Coimmunoprecipitation Co-IP.
An immunoprecipitation experiment designed to affinity-purify a bait protein antigen together with its binding partner using a specific antibody against the bait.
An affinity chromatography method that involves using a tagged or labeled bait to create a specific affinity matrix that will enable binding and purification of a prey protein from a lysate sample or other protein-containing mixture.
Monitor complex formation through transcriptional activation of reporter genes. Similar strategy to Western blotting with one key difference.
The antibody probe in a typical Western blotting detection is substituted with an labeled bait protein as the probe.
Tandem affinity purification-mass spectroscopy TAP-MS. TAP-MS is based on the double tagging of the protein of interest on its chromosomal locus, followed by a two-step purification process and mass spectroscopic analysis.
Microarray-based analysis allows the simultaneous analysis of thousands of parameters within a single experiment.